Co-Localization of ß-1,3-Glucanases and Callose During Somatic Embryogenesis in Cichorium
نویسندگان
چکیده
منابع مشابه
Regulation of callose synthases and β-1,3-glucanases during aphid infestation on barley cv. Clipper
Plant material and aphid species 8 RNA extraction and measurements of RNA concentration with Nano-Drop 9 RT-PCR and agarose gel electrophoresis 10 Real-Time PCR 11 Bioinformatics: searching for sequences, sequence alignment 13 Results 15 Expression of callose synthase 15 Callose synthase expression analysis with RT-PCR 17 Callose synthase expression analysis with Real-Time PCR 20 Expression of ...
متن کاملCallose Deposition Is Required for Somatic Embryogenesis in Plasmolyzed Eleutherococcus senticosus Zygotic Embryos
Dynamic changes in callose content, which is deposited as a plant defense response to physiological changes, were analyzed during somatic embryogenesis in Eleutherococcus senticosus zygotic embryos plasmolyzed in 1.0 M mannitol. During plasmolysis, callose deposition was clearly observed inside the plasma membrane of zygotic embryo epidermal cells using confocal laser scanning microscopy. The c...
متن کاملThree major somatic embryogenesis related proteins in Cichorium identified as PR proteins.
In Cichorium hybrid clone '474' (C. intybus L., var. sativum x C. endivia L., var. latifolia), the direct somatic embryogenesis process in leaf tissues is accompanied by an overall increase in the amount of proteins secreted into the culture medium. Amongst these, three major protein bands of 38 kDa, 32 kDa and 25 kDa were found in the conditioned media. These extracellular protein bands accumu...
متن کاملDevelopmental Localization and Methylesterification of Pectin Epitopes during Somatic Embryogenesis of Banana (Musa spp. AAA)
BACKGROUND The plant cell walls play an important role in somatic embryogenesis and plant development. Pectins are major chemical components of primary cell walls while homogalacturonan (HG) is the most abundant pectin polysaccharide. Developmental regulation of HG methyl-esterification degree is important for cell adhesion, division and expansion, and in general for proper organ and plant deve...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Signaling & Behavior
سال: 2007
ISSN: 1559-2324
DOI: 10.4161/psb.2.6.4715